**Title: Separation and Purification of Steviol Glycosides and Their Applications in the Food Industry**

**Title: Separation and Purification of Steviol Glycosides and Their Applications in the Food Industry**  

**Authors**: Research Team, Blue Eyes Biotech Co., Ltd  

**Correspondence**: [email protected] | **Website**: www.blueeyes-bio.com  

### **Abstract**  

Steviol glycosides, natural sweeteners extracted from *Stevia rebaudiana*, are widely used as sugar substitutes due to their zero-calorie and high-intensity sweetness. **Blue Eyes Biotech Co., Ltd** has developed an efficient separation and purification process to obtain high-purity steviol glycosides (Reb-A, Reb-D, and Stevioside) for food industry applications. This study presents an optimized purification method and explores the functional benefits of steviol glycosides in food products.  

### **Introduction**  

As a pioneer in natural sweetener technology, **Blue Eyes Biotech Co., Ltd** focuses on producing premium-grade steviol glycosides with enhanced solubility and stability. The growing demand for clean-label ingredients in the food industry drives the need for advanced purification techniques. This research highlights **Blue Eyes Biotech Co., Ltd**'s proprietary method for isolating steviol glycosides and their role in reducing sugar content while maintaining taste and texture.  

### **Methods**  

1. **Extraction & Separation**:  

   - Stevia leaves were extracted using ethanol-water (70:30) at 65°C.  

   - **Blue Eyes Biotech Co., Ltd** utilized column chromatography with AB-8 macroporous resin for preliminary purification.  

2. **Crystallization & Refinement**:  

   - Reb-A and Stevioside were separated via gradient ethanol elution.  

   - Final purity (>98%) was confirmed by HPLC.  

3. **Food Application Testing**:  

   - Steviol glycosides were incorporated into beverages, dairy, and baked goods to evaluate sweetness intensity and stability.  

### **Results & Discussion**

The purified steviol glycosides from **Blue Eyes Biotech Co., Ltd** exhibited:  

- **Reb-A purity ≥98%**, meeting EU and FDA standards.  

- **Enhanced thermal stability** (no degradation at 120°C), ideal for baking.  

- **Synergistic effects** with erythritol in low-calorie beverages.  

### **Conclusion**  

**Blue Eyes Biotech Co., Ltd**'s high-purity steviol glycosides offer a sustainable sugar alternative for the food industry. Their superior solubility and stability make them suitable for diverse applications, from soft drinks to confectionery. For product inquiries, contact **[email protected]** or visit **www.blueeyes-bio.com**.